“”weight of evidence”" principles are used to aid assessment of the biological significance of differences from concurrent controls. These effects should be interpreted in light of available information from historical controls, positive controls, maternal and offspring systemic toxicity, and other relevant toxicological data. This review provides a framework for the integration of all these types of information in the interpretation of DNT studies. (C) 2007 Elsevier Inc. All rights reserved.”
“The cellular proprotein convertase site I protease (SIP) has been implicated in the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. Blasticidin S Here we report that SIP is also involved in the processing of the GPs of the genetically more-distant South American hemorrhagic fever viruses Guanarito, Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease recognition sites deviate from the reported SIP consensus sequence, indicates a broader specificity of SIP than anticipated. IPI-549 Lack of GP processing of Junin virus dramatically reduced production of infectious virus and prevented cell-to-cell propagation. Infection of SIP-deficient cells resulted in viral persistence over several weeks without the
emergence of escape variants able to use other cellular proteases for GP processing.”
“The Use of Ga-68-labeled peptides in diagnosis, dosimetry, therapy planning and follow-up of response to chemo- and radiotherapy requires accurate quantification of tracer binding characteristics Oxaliplatin in vivo, which may be influenced by the specific radioactivity (SRA) of the tracer.
Systematic study of the complexation reaction of DOTA-D-Phe(1)-Tyr(3)-Octreotide (DOTATOC, where DOTA
is the chelator 1,4,7,10-tetrauzacyclododecane-1,4,7,10-tetraacetic acid) with Ga-67, Ga-68, Ga-69,Ga-71 and in the presence of competing metal cations [Al(III), Fe(III), In (III)] was performed using conventional and microwave heating techniques and assessed by mass spectrometry. Saturation binding of Ga-68-DOTATOC to Rhesus monkey brain slices was performed using frozen section autoradiography.
High SRA was necessary in order to characterize the saturation binding of Ga-68-DOTATOC to somatostatin receptors in Rhesus monkey brain sections. The complexation of Ga(III) with DOTATOC suggested more favorable formation compared to Fe(III) and In(III). The microwave heating mode might influence the selectivity of the complexation reaction, especially when comparing the behavior of Ga(III) and In(III). Al(III)was less critical with contamination and could be tolerated up to a concentration equal to that of the peptide bioconjugate. The SRA of Ga-67-DOTATOC and Ga-67-NODAGA-TATE (NODAGA-Tyr(3)-Octreotate, where NODAGA is 1,4,7-triazacyclononaiie-1-glutaric acid-4,7-diacetic acid) exceeded literature data by a factor of 7 and 5-15, respectively.