Figure 2 Effects of different doses of Guaifenesin on neuromuscular coordination in mice (N=6/group). Bar graphs represent mean±SEM of time spent
on the Rotarod for each group before and after the administration of Guaifenesin (G100-G400 mg/kg), 0.25% … Discussion Guaifenesin, a Propanediol drug used as an expectorant, showed an anticonvulsant effect in our animal model of seizure induced by PTZ. PTZ produces tonic–clonic convulsions in rats or mice and is commonly employed as a reliable animal model for screening new anti-epileptic drugs for absence seizure.21,22 We evaluated the anticonvulsant effects of Guaifenesin using PTZ-induced seizure Inhibitors,research,lifescience,medical in the present study, our results demonstrated that Guaifenesin could not only decrease the susceptibility of mice to PTZ-induced myoclonic, clonic, and especially tonic-clonic seizures but also protect the mice against PTZ-induced death. These results are in agreement with previous studies indicating that Propanediol drugs Inhibitors,research,lifescience,medical can exert anticonvulsant activity.23,24 Felbamate and Meprobamate are among Propanediol drugs previously shown to have anticonvulsant effects. Indeed, Felbamate is currently used as an anti-epileptic drug Inhibitors,research,lifescience,medical in clinical practice. Nonetheless, these drugs have serious side effects, including aplastic anaemia. This side effect is less likely to occur with Guaifenesin, which
makes it a good candidate as an anticonvulsant drug.
Additionally, Guaifenesin can be used during pregnancy and breastfeeding; this further underscores the desirability of this drug as a potential anticonvulsant in clinical practice. Be that as it may, future clinical trials should address its Inhibitors,research,lifescience,medical usefulness in absence seizure in humans. The mechanism by which Guaifenesin may exert anticonvulsant activity Inhibitors,research,lifescience,medical is not clear. However, animal models of epilepsy could partly predict the mechanism of action of some antiepileptic drugs.25 In a model of PTZ-induced seizure, the glutamatergic system, especially NMDA receptors, has been shown to play an important role.Thus, microdialysate, collected from hippocampal regions during seizures induced by PTZ, has revealed a rise in the concentration of mafosfamide glutamate.26 It has also been demonstrated that the administration of PTZ could up-regulate NMDA receptors in several regions of the rat brain.27 Therefore, it can be suggested that the NMDA antagonist activity of Guaifenesin may contribute to its anticonvulsant activity seen in this study. This INCB018424 chemical structure notion requires further elucidation in future studies. In concordance with previous studies,11 Guaifenesin at all the studied doses in the present investigation exhibited muscle relaxant activity as indicated by the findings of the Rotarod test, which raises the possibility that the effects of Guaifenesin against PTZ-induced seizure may be due to its muscle relaxant activity.