Electrophoresis

was done to the mixture NVP-BGJ398 purchase through 12% polyacrylamide gel for 6 hours LY2874455 at a constant 60 V. The gel was stained with Ethidium Bromide for 30 seconds and visualized on the gel documentation system. Any heteroduplex migrate more slowly through the gel as compared to its homoduplex counter parts. Sequence change could be detected by an extra band above the main homoduplex band. DNA sequencing of normal and mutated exons PCR samples showing variant bands as well as that of normal subjects were analyzed by direct DNA sequencing technique. Statistical analysis The data, either clinical or genetic findings, were statistically evaluated, interpreted and analyzed using the SPSS software version 16. Results Detected mutations Mutations were detected in 86.7% of the families (52 from total 60 families), in either BRCA1 or BRCA2. Of them 60% families were attributable to BRCA1 mutation and 26.7% families were attributable to BRCA2 mutations. They were identified by using the Selleck Geneticin combination of SSCP (Figures 1, 2, 3, 4 and 5) and heteroduplex analysis (Figures 6, 7). Four mutations were detected within the BRCA1 gene, and one mutation was detected in the BRCA2 gene. Eighty, from the total 120, asymptomatic relatives were mutation carriers. Figure

1 Single strand conformation polymorphism (SSCP) assay for exon 2 (BRCA 1) germline mutations. Lane N, normal female control. Lanes 1, 2, 3 and 4 show abnormal pattern of SSCP for patient, her

sister and her daughters. Lane M, 50 bp DNA ladder. Figure 2 Single strand conformation polymorphism (SSCP) assay for exon 22 (BRCA 1) germline mutations. Lane N, normal female control. Lanes 1, 2, 3 and 4 PDK4 show abnormal pattern of SSCP for patient, her sister and her daughters. Lane M, 50 bp DNA ladder. Figure 3 Single-strand conformation polymorphism assay for exon 13 (BRCA 1) germline mutations. Lane N, normal female control. Lanes 1, 2, 3 and 4 show abnormal pattern of SSCP for patient, her sister and her daughters. Lane M, 50 bp DNA ladder. Figure 4 Single-strand conformation polymorphism assay for exon 8 (BRCA 1) germline mutations. Lane N, normal female control. Lanes 1, 2, 3 and 4 show abnormal pattern of SSCP for patient, her sister and her daughters. Lane M, 50 bp DNA ladder. Figure 5 Single-strand conformation polymorphism assay for exon 9 (BRCA 2) germline mutations. Lane N, normal female control. Lanes 1, 2, 3 and 4 show abnormal pattern of SSCP for patient, her sister and her daughters. Lane M, 50 bp DNA ladder. Figure 6 Shows Heteroduplex analysis for germline mutations.