Zhao Y, Wei W, Lee IM, Shao J, Suo X, Davis RE: Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 2009, 59 (Pt 10) : 2582–2593.PubMedCrossRef 34. Powell R, Gannon F: Purification of DNA by

phenol extraction and ethanol selleck products precipitation. Oxford: Oxford University Press; 2002. 35. Bachem CWB, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RGF: Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development. Plant Journal 1996, 9 (5) : 745–753.PubMedCrossRef 36. Bachem CWB, Oomen RJFJ, Visser RGF: Transcript imaging with cDNA-AFLP: A step-by-step GSK872 order protocol. Plant Molecular Biology Reporter 1998, 16 (2) : 157–173.CrossRef 37. Bassam BJ, Caetanoanolles G, Gresshoff PM: Fast and Sensitive Silver Staining of DNA in Polyacrylamide Gels. Analytical Biochemistry 1991, 196 (1) : 80–83.PubMedCrossRef 38. Bananej K, Kheyr-Pour A, Hosseini Salekdeh G, Ahoonmanesh A: Complete nucleotide sequence of Iranian tomato yellow leaf curl virus isolate: further evidence for natural recombination amongst begomoviruses. Archives of GSK126 mouse virology 2004, 149 (7) : 1435–1443.PubMedCrossRef

39. Wu M: Development of a simple and powerful method, cDNA AFLP-SSPAG, for cloning of differentially expressed genes. Cobimetinib in vitro African Journal of Biotechnology 2006, 5 (24) : 2423–2427. 40. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic Local Alignment Search Tool. Journal of Molecular Biology 1990, 215 (3) : 403–410.PubMed 41. Martini M, Loi N, Ermacora P, Carraro L, Pastore M: A real-time PCR method for detection and quantification of ‘Candidatus Phytoplasma prunorum’ in its natural hosts. Bulletin of Insectology 2007, 60 (2) : 251–252. 42. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta

Delta C) method. Methods 2001, 25 (4) : 402–408.PubMedCrossRef 43. Torabi S, Wissuwa M, Heidari M, Naghavi MR, Gilany K, Hajirezaei MR, Omidi M, Yazdi-Samadi B, Ismail AM, Salekdeh GH: A comparative proteome approach to decipher the mechanism of rice adaptation to phosphorous deficiency. Proteomics 2009, 9 (1) : 159–170.PubMedCrossRef Authors’ contributions MGZ carried out the cDNA-AFLP experiments (including the extraction and reamplification of cDNA fragments) participated in sequence analysis, performed the real-time RT-PCR experiments, and contributed to data interpretation and manuscript writing. MM participated in in the analysis and interpretation of cDNA-AFLP data. SMA participated in plant sample preparation. NHZ, HRZ, and AA participated in sequence analysis, in interpretation of data, in automatic and Gene Ontology assignment.