We previously demonstrated that IC negatively regulates TLR4-triggered inflammatory

response in macrophages through FcγRIIb 27. We here demonstrate that in the presence of IC, FcγRIIb overexpression promotes resistance of immature DCs to TLR-triggered maturation induction and also increases DC tolerogenecity. Accordingly, IC-stimulated, FcγRIIb-overexpressing DCs (DC-FcγRIIb) can downregulate immune response more significantly both in vitro and in vivo, thus attenuating the progression of disease in lupus-prone mice. To investigate whether IC/Ig could inhibit TLR-induced maturation of DCs via FcγRIIb, Dinaciclib mw immature DCs derived from WT or FcγRIIb−/− mice were incubated with IC (OVA plus anti-OVA)/Ig (anti-OVA) for 24 h before these DCs were stimulated with LPS or CpG ODN for another 24 h. As for WT DCs, IC alone (whereas not Ig) slightly upregulated the expression of I-Ab, CD40, CD80

and CD86. Interestingly, IC pretreatment significantly inhibited the LPS or CpG ODN-induced upregulation Ferroptosis inhibitor of I-Ab, CD40, CD80 and CD86 expression, and Ig pretreatment significantly inhibited the LPS-induced upregulation of the four molecules and the CpG ODN-induced upregulation of CD80 and CD86 expressions (Fig. 1A). In contrast, neither IC nor Ig pretreatment had significantly inhibitory effect on the LPS or CpG ODN-induced upregulation of I-Ab, CD40, CD80 and CD86 expression on FcγRIIb−/− DCs (Fig. 1A). These data indicate that FcγRIIb mediates the inhibitory effect of IC/Ig above. IC alone could stimulate WT DCs as well as FcγRIIb−/− DCs to secrete TNF-α and IL-1β to some degree; however, IC pretreatment obviously suppressed LPS or CpG ODN-induced TNF-α and IL-1β secretion from WT DCs (Fig. 1B). In contrast, IC pretreatment could not suppress TNF-α and IL-1β secretion by LPS-stimulated FcγRIIb−/− DCs, and even promoted TNF-α and IL-1β secretion by CpG ODN-stimulated

FcγRIIb−/− DCs (Fig. 1B). OVA alone had no effect on the phenotype and cytokine Endonuclease secretion of WT DCs and FcγRIIb−/− DCs with or without stimulation with LPS, CpG ODN (data not shown). Considering that the Ig (anti-OVA mAb) we used, also exhibited inhibitory effect on LPS-induced DC maturation (in a similar manner to IC), we speculated that anti-OVA mAb might have some aggregated Ig, because aggregated Ig has a higher binding affinity to FcγRIIb than monomeric Ig. Therefore, we compared the differences between monomeric and aggregated Ig. As expected, the aggregated Ig significantly inhibited WT DCs to express I-Ab and CD40 and to secrete TNF-α in response to LPS stimulation, whereas monomeric Ig did not. However, neither aggregated Ig nor monomeric Ig had such inhibitory effect on LPS-induced FcγRIIb−/− DC maturation (Supporting Information Fig.