This

is in keeping with models of dental plaque development whereby the pathogenic potential alters as later colonizers become established [16]. A short format summary table of all data presented in this report can be found in Additional file 1. Additional files 2, 3, 4, 5, 6, 7 present the data in somewhat greater detail for each proteome quantitative comparison, including both raw and normalized spectral counts and associated statistics. Qualitative protein coverage information is summarized in Additional file 8. Additional file 9 shows a whole genome plot of the SgPgFn vs Sg comparison. Plots comparing spectral counts for technical replicates and spectral counts for each biological replicate are found in Additional file 10, as well as additional remarks about data reproducibility and the effects of normalization. The high correlations shown suggest that TPCA-1 cost the detected changes are due primarily to differences between the conditions being compared rather than random variability in the measurements. The original FileMaker™ database from which additional files 1, 2, 3, 4, 5, 6, 7, 8 were derived is available from the corresponding author. The raw data has been archived in a remote secure this website location as part of the University

of Washington’s lolo file retrieval system, and will also be made available through the United States Department of Energy’s Joint Genome Institute (JGI), and possibly other sites pending ongoing learn more discussions in the proteomics community with respect to best practices for permanent archival storage. Table 2 Relative abundance changes observed for the S. gordonii expressed proteome Comparison Unchanged Increased Decreased SgFn vs S. gordonii 421 188 (24%) 160 (21%) SgPg vs S. gordonii 389 212 (25%) 200 (26%) SgPgFn vs S. gordonii 287 163 (26%) 174

(28%) Bcl-w SgPg vs SgFn 375 161 (23%) 177 (25%) SgPg Fn vs SgFn 327 111 (19%) 146 (25%) SgPg Fn vs SgPg 556 15 (2%) 56 (9%) Energy metabolism and sugar transport Changes to pathways for energy metabolism and sugar transport in the multispecies communities were consistent with a higher level of available energy metabolites and a lower pH. Oral streptococcal species primarily derive their energy from the breakdown of carbohydrates. Figures 2, 3, 4, 5, 6, 7 compare energy metabolism pathway proteins between the different communities (2 SgFn vs Sg, 3 SgPg vs Sg, 4 SgPgFn vs Sg, 5 SgPg vs SgFn, 6 SgPgFn vs SgFn, 7 SgPgFn vs SgPg). Compared to Sg alone the multispecies communities showed increased levels for both the glycolysis pathway and the pentose phosphate pathway, implying higher energy availability (Figures 2, 3, 4). The presence of Pg appeared to be dominant as SgPgFn was very similar to SgPg (Figure 7). Even though both pathways were increased in the presence of Fn or Pg there was a difference in emphasis (Figure 5). Sg in contact with Pg had larger increases in the glycolysis pathway while Sg with Fn had larger increases in the pentose phosphate pathway.