The majority used on a cross-sectional design, IWR-1 price with only three studies utilising a cohort and two a case–control design. While 17 studies used population-based survey data or baseline data of ongoing trials, eight studies were based on clinical samples of women from one to 115 health facilities. The definitions used to assess ‘early sexual debut’ varied substantially between studies. Some studies defined early

sexual debut as the sexual debut occurring before the age 14, while others used 19 as their cut-off age. In addition, several studies measured age at first sex continuously or using more than one age intervals. As a result, for example, they compared the risk of HIV infection of women who had their sexual debut before the age of 15 to that of women whose sexual debut was after the age of 25, and not to that of women who had their first sex at the Selleckchem Ribociclib age of 15 or afterwards. Of the 25 studies included in this review, none was rated to have a high quality, seven to have medium quality, 13 to have low quality and five to have very low quality. Study sites included South Africa (six sites), Zimbabwe (six sites), Tanzania (four sites), Cameroon (three sites), Kenya (two sites), Rwanda (two sites), Malawi (one site), Nigeria (one site), Ghana (one site),

and one study was a four-city study in Cotonou, Benin, Yaounde, Cameroon, Kisumu, Kenya and Ndola, Zambia. Of the 26 results in the 23 articles, which reported unadjusted associations Montelukast Sodium between early sexual debut and women’s increased HIV infection risk, 13 found a significant association. As can be seen in Table 2, if studies that measured age at first sex as a continuous variable are not considered in the analysis, 12 of 21 found a significant association. Similarly, if only studies with a sample size above 300 are considered, 13 of 25 found a significant association. Importantly, all five studies with a sample size above 3000 found a significant association between early sex and HIV infection. In addition, among those studies with at least a medium quality score, five of seven studies report a significant unadjusted association between

early sexual debut and women’s increased HIV risk. In practice, in the studies reviewed, different authors controlled for different variables in subsequent multivariate analyses. Studies controlling for duration of sexual activity, women’s sexual risk behaviour, partner’s higher HIV infection risk and socio-demographic variables will be discussed separately. Surprisingly, only two studies, both from Zimbabwe and both of medium quality, controlled for women’s duration of sexual activity in their multivariate analysis (Table 3). In both cases, the association remained significant, suggesting that women who start sex at a young age are not solely at increased HIV risk because they are simply exposed to HIV risk for longer by being sexually active.