The increasing number of Chinese citizens working in central Africa, especially in rural areas, means that presentations with loiasis can be expected to increase in China within the

next few years. In this instance, the diagnosis was made using a PCR technique applied on the extract from a biopsy of a Calabar swelling. Previous studies have shown that serological tests by ELISA are able to detect microfilaremia EMD 1214063 and filarial antigens or antifilarial antibodies.[1, 7] However, in this case, the infecting filarial species could not be identified because of extensive antigenic cross-reactivity. Nested PCR using DNA extracted from blood as a template has been reported as a specific method and also had a 95% sensitivity in detection of occult loiasis.[1] Although not widely used, nested PCR provides an alternative diagnostic measure for loiasis when clinical features are not typical and parasites cannot be removed directly from tissue or blood samples. This case also provides verification that Calabar swellings are manifestations of localized angioedema that are probably related to the subcutaneous migration of L loa. Ivermectin is a safe and popular choice for treatment of filariasis,[8] partially selleckchem because of its inability to penetrate the blood–brain barrier.

However, ivermectin has a minor effect on adult parasites and patients need retreatment annually. Unfortunately, this drug is not available in China; therefore, DEC, a piperazine derivative with activity against both microfilariae and adult worms of L loa, was used. According to the proposed treatment strategy, DEC can only be administered after having checked the level of microfilaremia,

and it is most suitable for patients where the microfilarial density is below 2,000 mf/mL.[9] Although microfilaremia was not detected in this patient, physicians and technicians in areas where L loa is not endemic may not be experienced in recognizing the microfilariae under the microscope Cyclin-dependent kinase 3 and DEC was administered. Patients with high loads of L loa microfilariae may experience serious adverse events including shock, encephalitis, and hemorrhage following the use of DEC because of rapid killing of the microfilariae,[10] and severe encephalopathy was reported recently in a patient with low microfilaremia (0.7 μL−1).[11] Our patient received prednisone at the start of therapy and reported no drug-related adverse reactions. In summary, we report a case of loiasis in a male returning from working in Equatorial Guinea, which was diagnosed by nested PCR using DNA extracted from tissue. The authors are grateful to Professor J. Iredell and Dr S. Partridge, Center of Infectious Diseases and Microbiology, Westmead Hospital, The University of Sydney, Australia, for the critical reading of this article.