The cIEF method was particularly useful for this study since it is able to separate multiple forms of the same protein, provided they have altered pIs. There are, however, some limitations to this technique. The IEF step is very sensitive to detergents and salts and this limits the types of homogenization buffers that can be used. The antibodies used for detection must be capable of detecting either native or urea-denatured Navitoclax ic50 forms of the protein and not all antibodies that work for western blot work for cIEF. Furthermore, the factors that promote efficient crosslinking of the protein to the capillaries are only poorly understood, and it is possible

that some species are missed entirely. Finally, at the present time the technique is not preparative and direct analysis of the peaks, for example by MS, is not possible. Nonetheless, cIEF is well suited to detect many of the common protein PTMs involved in regulation of protein function. A schematic representation of the effects of HCV and ethanol on FOXO3 species is illustrated in Fig. 8. Under normal conditions, the majority of FOXO3 is in the

nucleus forming several species that differ in the presence of PTMs including phosphorylation, acetylation, and ubiquitination. Quizartinib clinical trial In the cytosol, FOXO3 formed more acidic species. Under control conditions, all FOXO3 species were arginine methylated. The effect of HCV infection was to translocate FOXO3 to the nucleus and activate its 上海皓元 transcriptional activity. In the nucleus, HCV-activated JNK phosphorylation of FOXO3 on S-574, and possibly other residues, and formed a novel FOXO3 species with a pI of 5.85. Serine-574 was absolutely necessary for HCV- or JNK-mediated FOXO3 activation and its phosphorylation resulted in the conversion of the pI 5.97 FOXO3 nuclear species to a more acidic one. While JNK-induced S-574 phosphorylation was necessary, it was probably not sufficient for the all the HCV-induced changes. We were able to duplicate the formation of the 5.85 FOXO3

nuclear peak with active JNK1 expression but not the other HCV-induced modifications that affect FOXO3 and produce a pI 6.62 peak. Furthermore, the addition of a single phosphate by itself should only shift FOXO3 pI by ∼0.04 pH units. The generation of the 5.85 species with its acidic shift of 0.15 pH units thus likely involves either significant conformational changes or other modifications such as changes in ubiquitination. The importance of JNK, however, is consistent with the literature on other FOXOs as JNK plays a role in the oxidative stress dependent activation of FOXO4 by phosphorylation of T447 and T551.[26] Human liver specimens from HCV-infected patients similarly showed the presence of the HCV-specific 5.85 species of FOXO3. This species was not present in either normal livers or livers from patients with NASH.