Results: Hedgehog signaling was abnormal activated in a ligand-independent manner in the process of gastric tumorigenesis. Overexpression of Gli1 and poorexpression of SuFu were typical events in gastric cancer tissues. Gli1 overexpression was correlated with poor differentiated histology, advanced clinical stage, membrana serosa infiltration and lymph node metastasis in
patients with gastric cancer. The data of mutiple cell biological assays showed human gastric cancer cells required active Hedgehog signaling for cell suvival, proliferation, migration and colony formation. N-Shh treatment significantly enhanced cell migration and colony formation of gastric cancer cells. Moreover, the results of cDNA microarray analysis indicated after RXDX-106 cell line treatment of cyclopamine or GANT61 (inhibitors of Hedgehog signaling), Differentially Expressed Genes (DEGs) in gastric cancer cells were enriched in apoptosis and MAPK pathway. Hedgehog pathway inhibitors suppressed gastric cancer cell growth via inducing apoptosis. Conclusion: These findings demonstrate vital role of activated Hedgehog signaling pathway in promoting gastric tumorigenesis and development. Hedgehog signaling pathway may be a target
of gastric cancer therapy. Key Word(s): 1. Hedgehog signaling; 2. gastric cancer; 3. Gli1; 4. Metformin cDNA microarray; Presenting Author: XIAOLEI SHI Additional Authors: JUN TIE, SIJUN HU, YONGZHAN NIE Corresponding Author: XIAOLEI SHI, YONGZHAN NIE Affiliations: xijing hospital of digestive diseases; Methocarbamol xijing hospital of digestive diseases; xijing hospital of digestive diseases; xijing hospital of digestive diseases Objective: MicroRNAs(miRNAs) are small non-coding RNAs(ncRNAs). Its control multiply processes. A number of miRNAs that are associated with gastric cancer have been identified to date. Based on literatures, we predicted the altered expression of miR-148a/b in gastric
cancer, and may Play a critical Part in carcinogenesis. Our previous study showed that miR-148a/b were down-regulated in gastrointestinal cancer and miR-148a/b inhibit gastric cancer invasion and metastasis. PrPc may be a target of miR-148a/b. In the Present study, we try to unravel the function and mechanism of miR-148a/b in gastric cancer. Methods: The function of gastric cancer cells (MKN28, SGC7901) with overexpression of miR-148a/b was measured in proliferation, migration and invasion. We analyzed the expression of miR-148a/b and PrPc mRNA in cell lines by qRT-PCR. Then we observed the PrPc mRNA and protein levels in MKN28 and SGC7901 cells with overexpression of miR-148a/b. The relationship between miR-148a/b and PrPc expression was further investigated by in situ hybridization and immunohistochemistry in 90 cases of GC and matched adjacent normal tissues. We constructed a luciferase reporter to test whether PrPc is functionally regulated by miR-148a/b.