GSK690693 mouse metabolites with significant differences in quantity between (C) and (D) are labeled. Relative contents Relative contents 104/106     (sec) in 104spores/ml* in 106spores/ml* ratio TCA cycle intermediates Malic acid 587.5 0.0187 ± 0.0001 0.0098 ± 0.0027 1.91 Fumaric acid 1349.0 0.0869 ± 0.0090 0.0509 ± 0.0043 1.71 Succinic acid 269.8 0.0103 ± 0.0020

0.0043 ± 0.0016 2.4 Amino acids Glycine 417.0 0.0066 ± 0.0028 0.0034 ± 0.0003 1.94 Phenylalanine 713.7 0.1649 ± 0.0330 0.0084 ± 0.0007 19.63 Proline 410.0 0.0552 ± 0.0179 Tozasertib nmr 0.0099 ± 0.0009 5.58 Valine 331.5 0.0191 ± 0.00028 0.0088 ± 0.0006 2.17 Tyrosine 964.1 0.0430 ± 0.0090 0.0242 ± 0.0027 1.78 Isoleucine 404.0 Milciclib research buy 0.0059 ± 0.0017 0.0042 ± 0.0003 1.4 Fatty acids Palmitic acid 1054.5 0.3447 ± 0.037 0.2640 ± 0.011 1.31 Stearic acid 1212.4 0.2218 ± 0.027 0.1402 ± 0.0133 1.58 Oleic acid 1193.0 0.0833 ± 0.0024 0.0744 ± 0.0033 1.12 Linoleic acid 1188.8 0.8959 ± 0.0671

0.6315 ± 0.0554 1.42 Nucleotides Pyrimidine 449.0 0.0154 ± 0.0044 0.0039 ± 0.0004 3.95 Purine 630.8 2.1901 ± 0.3141 1.3194 ± 0.0221 1.66 AA metabolism Pentanediamine 881.1 0.1703 ± 0.0143 0.0162 ± 0.0011 10.51 Amine 369.0 0.0734 ± 0.0261 0.0328 ± 0.0036 2.24 Sugar metabolism Ribitol 784.2 0.6276 ± 0.1768 2.6039 ± 0.1502 0.24   Glucopyranoside 1561.8 0.1130 ± 0.0198 0.3344 ± 0.0354 0.34   Gluconolactone-6-P 821.6 0.5679 ± 0.0839 0.7094 ± 0.0181 0.80   Glycerol 986.0 0.0073 ± 0.0015 0.0103 ± 0.0009 0.71   Butanediamine 801.1 0.0656 ± 0.0086 0.1224 ± 0.0051 0.54   Ehylamine 563.0 0.2082 ± 0.0320 0.2436 ± 0.0013 0.85   Galactose 948.1 1.6122 ± 0.4037

1.9547 ± 0.3306 0.82 *Values represent metabolites abundances (mean ± SD) of 3 replicates from mixed 3 independent samples, as measured by GC-Tof-MS in 3-day mycelia (normalized Farnesyltransferase by C17:0 fatty acid). R.T.: retention time; TCA: tricarboxylic acid; AA: amino acid. Addition of TCA cycle intermediates did not affect AF biosynthesis To test if reduced TCA cycle intermediates in mycelia are the primary cause of reduced AF biosynthesis in the high initial spore density culture, malic acid, fumaric acid and succinic acid were added to the PMS medium at the concentrations of 0.5 mM or 5 mM, and 0.5 or 5 mM NaCl was added to the culture as a control, and then performed liquid incubation with the final spore densities of 104 or 106 per ml using freshly prepared A. flavus A3.2890 spore suspensions. TLC analyses were performed for AFs extracted from the media. We observed that none of these treatments had any significant effect on AF production. No AF production was observed in any of the high initial spore density cultures (Figure 7).