MuSK knock-out mice also displayed presynaptic defects in addition to postsynaptic ones, indicating that MuSK is required for retrograde signals, so far unidentified, to maintain the pre-synaptic structure

#Paclitaxel price randurls[1|1|,|CHEM1|]# in mature NMJ. Figure 2 Schematic appearances of NMJs observed in normal volunteers and patients. A: Normal NMJ. AChRs are concentrated at the peaks of abundant, well-preserved and intricately twisted junctional folds. B and C: NMJ in EAMG induced by MuSK antibodies, CMS with … Resemblance of clinical features between MuSK MG and CMS with Dok-7 mutations Recently a MuSK-interacting protein called Dok-7 was discovered (25) and Inhibitors,research,lifescience,medical Inhibitors,research,lifescience,medical identified as a member of the Dok family of cytoplasmic proteins. Dok-7 is postulated to have

three main functional domains: a pleckstrin homology (PH) domain, essential for membrane association; a phosphotyrosine-binding (PTB) domain involved in the Dok-7 induced activation of MuSK; and a large C-terminal Inhibitors,research,lifescience,medical domain containing multiple tyrosine residues. Dok-7 knock-out mice showed marked disruption of neuromuscular synaptogenesis that was indistinguishable from the features found in MuSK-deficient mice. Thus, Dok-7 is essential for neuromuscular synaptogenesis through its interaction with MuSK. Mutations in the Dok-7 protein cause a genetic form of limb-girdle myasthenia (also classed as CMS) (26). Some clinical features in these patients resemble those in the severe type of MG accompanied by MuSK antibodies (27). Proximal muscles are usually more affected than those in Inhibitors,research,lifescience,medical distal regions, as evident in MuSK MG patients, and ptosis is often present. Limb-muscle weakness is comparatively less severe. Previous studies showed no reduction of AChR clustering with significant changes in NMJ of MuSK MG patients (20),

but further structural analysis of NMJ is required Inhibitors,research,lifescience,medical in muscles where severe weakness occurs commonly. The weakness and atrophy are not observed uniformly in muscles of these patients, although both MuSK and Dok-7 are essential for the formation of NMJ during the embryonic stage Sclareol (25). Of course, one of the major distinctions between acquired MuSK MG and CMS with the Dok-7 mutation is the timing when weakness begins. The CMS patients typically have difficulty in walking after reaching that normal motor milestone during early childhood, whereas the onset of weakness for MG patients, in most instances, occurs in adulthood. Interestingly, AChR clustering and post-synaptic folds are reduced with small motor terminals as observed at NMJ in CMS with Dok-7 mutations. AChR clustering and post-synaptic folds are reduced with small motor terminals as observed at NMJ in CMS with Dok-7 mutations (28).