Moreover, no association of p28GANK with those regulators was observed by immunoprecipitation
in MHCC-97L cells (data not shown). Through coimmunoprecipitation, we detected a complex consisting of p28GANK, RhoGDIα (RhoGDIa (Rho GDP dissociation inhibitor (GDI) α), and RhoA (ras homolog gene family, member A) proteins (Fig. 5E), which results in repression of ROCK2 (Rho-associated, http://www.selleckchem.com/products/LDE225(NVP-LDE225).html coiled-coil containing protein kinase 2) activity (Supporting Information Fig. 6). This result suggests a mechanism in which p28GANK activates p-AKT signaling through control of the RhoGDIα/RhoA/ROCK2 pathway. Consistent with this, Man et al. reported recently that AZD1208 cost p28GANK promoted
RhoGDIα interaction with RhoA, leading to inhibition of RhoA/ROCK2 activity, and prolonged AKT activation in NIH3T3 cells, human embryonic kidney 293 cells, and lung cancer cells.23 We further analyzed the expression levels of p28GANK, p-AKT, E-cadherin, TWIST1, HIF-1α, RB, and p53 in clinical HCC samples. Tissue microarray analysis of 130 patient specimens revealed a strong correlation of p28GANK expression with p-AKT levels (r = 0.2505, P = 0.0004) (Fig. 6A). Moreover, patients whose tumors expressed above-average levels of p28GANK or p-AKT exhibited significantly decreased trend in any of the prognostic indicators, including time to DFS and OS due to HCC-related death (Supporting Information Fig. 7A,B). For patients whose tumors had above-average levels of both p28GANK and p-AKT, adverse outcomes were exacerbated (Fig. 6B). Using the combination of these two parameters increased the prognostic value, as compared to p28GANK or p-AKT overexpression alone (Fig. 6B; Supporting Information Fig. 7A,B). In conclusion, evaluation of both p28GANK expression and p-AKT signal is a powerful predictor of poor prognosis, further supporting a model of p28GANK activation of PI3K–AKT–HIF-1α signaling, resulting in EMT
occurrence, learn more VEGF and MMP2 production, and thus metastases of HCC cells (Fig. 6C). Efforts to elucidate the molecular mechanism underlying HCC tumorigenicity, invasion, and metastasis of HCC are warranted in order to identify biomarkers for prediction and intervention. In this study, we determined the significance and underlying mechanism for p28GANK overexpression in HCC progression and metastasis. The p28GANK content was low in normal hepatocytes, increased in noninvasive and primary HCC cells, and reached the highest level in invasive HCC and PVTT cells. This progressively increased expression profile paralleled with deterioration of the disease, suggesting a role of p28GANK in progression of HCC.