It also provides biology-founded ammunition in favor of the controversial argument that microbial diagnostics have a place in the decision-making and therapeutic management of patients with periodontitis [46]. Finally, we emphasize that the subject sample involved in the present study included both chronic and aggressive periodontitis patients and subjectsbelonging to various race/ethnicity groups. It is conceivable that the typeof disease and race/ethnicity-related charactersitics may be additional determinants of the gingival tissue transcriptome and/or may act asmodifiers of the association between bacterial
colonization patterns andtissue gene expression. CHIR-99021 mouse We intend to explore these possibilities insubsequent reports. Conclusion Using data from 120 patients, 310 gingival tissue samples and the adjacent 616 subgingival plaque samples, we demonstrate a strong correlation between the bacterial content of the periodontal pocket and the gene expression profile of the corresponding gingival tissue. The findings indicate that the subgingival bacterial load by several – but clearly not all – investigated periodontal species may determine gene expression in the adjacent selleck compound gingival tissues. These cross-sectional observations may serve
as a basis for future longitudinal prospective studies of the microbial etiology of periodontal diseases. Acknowledgements This work was supported by grant DE015649 and a CTSA Award RR025158 (P.N.P.). Additional support was provided by K99 DE-018739 (R.T.D); GM076990, a Michael Smith Foundation for Health Research Career Investigator Award, and an Award from the Canadian Institutes of Health Research (P.P); DE16715 (M.H.); Neue Gruppe Wissenschaftsstiftung, Wangen/Allgäu, Germany and IADR/Philips Oral Healthcare Young Investigator Research Grant (M.K). Electronic supplementary material Additional file 1: Table S1. Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of A. actinomycetemcomitans in the adjacent pockets.
(ZIP 3 MB) Additional file 2: Table S2. Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of P. gingivalis in the adjacent pockets. (ZIP 3 MB) Additional file 3: Table S3. Cytidine deaminase Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of T. forsythia in the adjacent pockets. (ZIP 3 MB) Additional file 4: Table S4. Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of T. denticola in the adjacent pockets. (ZIP 3 MB) Additional file 5: Table S5. Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of P. intermedia in the adjacent pockets. (ZIP 3 MB) Additional file 6: Table S6. Statistically significantly differentially expressed probe sets in the gingival tissues according to levels of F.