In red deer and fallow PHA-848125 mouse deer, one piece of the tonsils and head lymphnode samples, always containing at least half left and half right medial retropharyngeal lymph node, were submitted for culture. Due to logistic and budget constraints, no thoracic or abdominal lymphoid tissues were cultured except when TB-compatible macroscopic lesions were evidenced. Table 1 Mycobacterial identification and molecular typing results by
species and sampling site within Doñana National Park (DNP), Spain (CR Coto del Rey; SO Los Sotos; EB Estación Biológica; PU El Puntal; MA Marismillas; see Figure 1 on molecular typing patterns and Figure 6 on regions within DNP). Mycobacteria Other Than Tuberculosis (MOTT) Mycobacterium bovis Host Site n M. scr. M. int. M. xen. M. int. Total MOTT A1 A3 B2 B5 C1 D4 E1 F1 Total M. bovis Wild boar CR 14 12 1 13 SO 18 3 3 8 2 10 EB 31 2 6 3 11 5 2 7 PU 29 1 5 6 7 12
19 MA 32 5 7 1 13 Total 124 6 6 8 20 37 21 1 2 1 62 Red deer CR 35 8 1 1 10 SO 35 6 1 7 8 1 9 EB 12 1 1 2 1 3 PU 3 1 1 2 MA 10 1 1 Total 95 6 3 9 19 1 2 1 CHIR99021 1 24 Fallow deer CR 36 2 2 7 1 8 SO 35 9 1 10 8 2 10 EB 9 3 1 4 2 2 PU 5 2 2 1 1 MA 15 Total 100 16 1 1 18 18 3 21 TOTAL 319 28 6 1 12 47 74 1 23 1 1 1 5 1 107 M. scr. = Mycobacterium Loperamide scrofulaceum; M. int. = Mycobacterium interjectum, M. xen. = Mycobacterium xenopi, M. int. = Mycobacterium intracellulare Table 2 Infection with Mycobacterium bovis, Mycobacteria Other Than Tuberculosis (MOTT), or M. bovis/MOTT co-infection in wildlife hosts from Doñana National Park, Spain. MOTT pos MOTT neg Host M. bovis pos M. bovis neg M. bovis pos M. bovis neg Red deer 1 8 26 60 Fallow deer 3 15 19 63 Wild boar 4 16 57 47 Figure 1 Doñana National Park, Spain. Park boundary is marked by a solid line. From north to south: CR Coto del
Rey; SO Los Sotos; EB Estación Biológica; PU El Puntal; MA Marismillas. Shadowed areas are marshlands used as cattle pastures (Marisma de Hinojos and Las Nuevas). Symbols show sampling sites for wild boar (squares), fallow deer (circles) and red deer (Cobimetinib in vivo triangles). Social groups were defined as animals sampled the same day at the same site, and with characteristics that were compatible with forming a stable (e.g. female-yearling) or seasonal (e.g. rut mixed) group. Only part of the individuals belonging to a given social group was sampled. Sampling was performed according to European (86/609) and Spanish laws (RD 223/1988; RD 1021/2005), and current guidelines for ethical use of animals in research (ASAB, 2006) and UCLM animal experimentation committee.