In addition to oligodendrocytes, modest

astrocyte production was also reported in some but not all of these studies—the main source of reactive astrocytes being preexisting astrocytes, not NG2-glia. One study does not conform to this pattern. This is a study of cell generation following a cold-induced injury to the cerebral cortex (Tatsumi et al., 2008), in which the major product of NG2-glia appeared to be protoplasmic “bushy” astrocytes, not oligodendrocytes (see below). NG2-glia derived neurons were not found in any of these studies, however. The main features of all the fate-mapping studies discussed in this review are summarized in Table 1. Following a cortical (gray matter) stab injury in adult Olig2-CreER∗: Z/EG mice, Dimou et al. (2008) reported oligodendrocyte generation but little or no astrocyte production. An accumulation of GFAP+ BrdU+ reactive astrocytes Lapatinib chemical structure was found in the vicinity of the lesion, as expected, but these were mostly reporter-negative (i.e., not NG2-glia derived). Very similar results to these were reported following cortical stab wounds in NG2-CreER∗: Rosa26-YFP AUY-922 manufacturer mice ( Komitova et al., 2011). A subsequent BrdU fate mapping study ( Simon et al., 2011) failed to find

evidence for any astrocyte production from dividing NG2-glia after cortical stab injury. The emerging Adenylyl cyclase consensus from these studies is that the reactive (hypertrophic, strongly GFAP+) astrocytes that form the glial “scar” around sites of injury in the cortex are derived predominantly or exclusively from pre-existing astrocytes, not from NG2-glia. This conclusion has been supported by complementary

experiments in which astrocytes were labeled specifically by injecting a GLAST-CreER∗ lentiviral vector into the cortex of reporter mice, and their fates followed before and after cortical stab injury ( Buffo et al., 2008). Before injury, the labeled astrocytes were quiescent (did not incorporate BrdU after a long label) but, after injury, they started dividing and generated many new astrocytes, but not other cell types, at the site of the wound. This also seems to be what happens after spinal cord injury. Barnabé-Heider et al. (2010) made a transverse cut through the dorsal funiculus of the spinal cord, severing the ascending and descending axon tracts. They observed new oligogenesis but insignificant astrocyte production from NG2-glia (marked using Olig2-CreER∗), despite a robust astrocytic reaction/gliosis. Most interestingly, they identified two separate components of the astrocytic reaction—a localized accumulation of GFAP+ astrocytes at the core of the lesion site in the dorsal funiculus and a more diffuse accumulation/gliosis around the lesion site and throughout the spinal cord at the level of the injury.