Genotypes: y w hsFLP/yw, GrinCherry /, FRT82 ubiGFP/FRT82 rin2 y w hsFLP/yw, GrinCherry /, FRT82 ubiGFP/FRT82 PGawBrinNP3248 y w hsFLP/yw, GrinCherry /, FRT82 ubiGFP/FRT82 PGawBrinNP5420 y w eyFLP/ yw, FRT82 cl w /FRT82 y w eyFLP/yw, FRT82 cl w /FRT82 PGawBrinNP3248 y w eyFLP/yw, FRT82 cl w /FRT82 PGawBrinNP5420 yw, FRT82/FRT82 FMR1D50M PGaw BrinNP3248 yw, FRT82 FMR1D113M/FRT82 PGawBrinNP3248 yw, FRT82 FMR1D113M rin2/FRT82 PGawBrinNP3248 yw eyFLP/yw, FRT82 FMR1D113M rin2/FRT82 FMR1D50M PGaw BrinNP3248 y w eyFLP/yw, GrinCherry /, FRT82/FRT82 FMR1D50M PGawBrinNP3248 y w eyFLP/yw, GrinCherry / , FRT82 FMR1D113M rin2/FRT82 FMR1D50M PGawBrinNP3248 yw, FRT82/FRT82 FMR1D50M PGawBrinNP5420 yw /yw, FRT82 FMR1D113M/FRT82 PGawBrinNP5420 yw /yw, FRT82 rin2/FRT82 FMR1D50M PGawBrinNP5420 y w eyFLP/yw, FRT82 FMR1D113M rin2/FRT82 FMR1D50M PGawBrinNP5420 yw, GrinCherry /, FRT82/FRT82 FMR1D50M PGaw BrinNP5420 y w eyFLP/yw, GrinCherry /, FRT82 FMR1D113M rin2/FRT82 FMR1D50M PGawBrinNP5420 y w eyFLP/yw, FRT82 cl w /FRT82 y w eyFLP/yw, FRT82 cl w /FRT82 FMR1D113M rin2 y w eyFLP/yw, Grin /, FRT82 cl w / FRT82 FMR1D113M rin2.
Figure S6 CaprRNAi strongly additional hints reduces Capr levels. CaprRNAi overexpressing clones in eye imaginal discs of third instar larvae lower Capr levels as judged by Capr staining. Scale bar represents 50 mm. Genotypes: y w hsFLP/yw, UAS CaprRNAi/, Act. CD2. Gal4, UAS GFP/. Figure S7 Lig regulates Rin levels but not at the protein level. lig overexpressing clones display enhanced levels of Rin Cherry expressed from the GrinCherry transgene. Scale bar represents 50 mm. Negatively marked 72 h old lig1 mutant clones in eye imaginal discs of third instar larvae expressing UAS HA rin beneath the control of ey and GMR Gal4.
Note that HA Rin is even more selleck chemicals Raf Inhibitors strongly expressed within the posterior portion in the disc as a consequence of the robust expression of Gal4 by GMR Gal4. Scale bar represents 50 mm. Genotypes: y w hsFLP/yw, GrinCherry /, Act. CD2. Gal4, UAS GFP/UAS lig y w hsFLP/yw, FRT42 ubiGFP/FRT42 lig1; ey Gal4, GMR Gal4/UAS HA rin. Figure S8 Lig will not regulate bantam miRNA, EGFR signaling, Myc, Hippo signaling, Insulin signaling, Wnt signaling and Hedgehog signaling. lig1 mutant clones in eye imaginal discs of third instar larvae usually do not display an upregulation of a minimal Hippo response element. Scale bar represents 50 mm. Negatively marked 72 h old lig3 mutant clones, B and B) and lig overexpressing cells marked with RFP in eye imaginal discs of third instar larvae usually do not influence on a bantam miRNA reporter.
Scale bar represents 50 mm. Negatively marked 72 h old lig1 mutant clones ) in wing or eye imaginal discs usually do not modify expression or localization of pnt lacZ, pAkt, Myc, Dll, Sens, Ptc and Cut.