Furthermore, molecular weight calculator as indicated previously, the amount of the exogenously supplied CK script abundance in salt treated WT A. thaliana lines. Two of them were among highly down regulated genes in both salt treated ABR17 transgenic line and WT A. thaliana lines. Our qRT PCR data showed sim ilar trend as observed by microarrays for all the above mentioned genes in both salt treated ABR17 transgenic and salt treated WT microarrays. From these results, it is apparent that all the four genes showed the same trend both in our microarray analysis and qRT PCR studies although the absolute values were different with these two experimental methods. The gene XTR6 was selected because it was among one of the most highly induced transcripts of any gene on our salt treated ABR17 transgenic A. thalina microarray.

The genes bHLH, RAP 2. 6 and ATNAC3 were chosen because their expression was the highest among any other transcription factors identified in Inhibitors,Modulators,Libraries response to salt in ABR17 transgenic line. The genes At4g25810, At5g43650, At1g43160 and At3g15500 showed transcript abundance of 5. 10, 5. 29, 3. 19 and 3. 88 fold, respectively in microarray analysis of salt treated WT A. thaliana, while our qRT PCR analysis of salt treated WT A. thaliana showed transcript abundance of 32. 51, 14. 17, 6. 58 and 10. 23 fold. Our microarray analysis and qRT PCR Inhibitors,Modulators,Libraries results showed the similar trend in both salt treated ABR17 and WT samples, The genes PDF1. 2a, EXPL1, GRP, and MAPK 11 were chosen as these were val idated in our first set of microarrays.

Once again, a similar trend was observed between microarrays and qRT PCR analysis thus validating our microarray results. Relative expression of CK biosynthetic Inhibitors,Modulators,Libraries genes in ABR17 transgenic A. thaliana As discussed earlier, our observations indicated that many of the Inhibitors,Modulators,Libraries genes identified in the transgenic plants as being up regulated are from families Inhibitors,Modulators,Libraries that contain CK responsive members. We have also previously reported higher endog enous concentrations of CK in this line, which sug gested the possibility that this may be due to either enhanced de novo CK biosynthesis or decreased degrada tion. Specifically, the endogenous concentration of total CK in the transgenic line used in this study was 1 3 fold higher, with the concentration of zeatin being 1. 4 fold and IP being 2 fold higher in these transgenic lines.

However, we did not detect any IPT or CKX genes as being significantly up or down reg ulated genes in our microarray experiments suggesting that the elevated http://www.selleckchem.com/products/crenolanib-cp-868596.html endogenous CK concentrations previ ously reported may not be the result of increased or decreased activities of IPT and CKX genes, respectively. In order to confirm our microarray results and to lend addi tional support to our above mentioned hypothesis with respect to the roles of IPT and CKX expression in ABR17 transgenic A. thaliana, we also per formed qRT PCR analysis of the expression of IPT and CKX genes using qRT PCR.