fastigiatum and AT4G25530, AT2G19520, and AT1G31812 in P. enysii. The gene XERO2 was only identified up regulated in P. fastigia tum within the microarray evaluation but not with tag profiling. These success propose that, even though genes implicated in cold tolerance were up regulated in each species, the re sponse to cold was extra significant in P. fastigiatum. A process not detected from differential expression or ontology analyses in the heterologous microarrays, but detected by tag profiling was flower induction. AT4G31120, a gene that promotes flowering by repressing flowering locus C was up regulated in P. enysii in all datasets except A0 and A1. FLC was up regulated in P. fastigiatum in all datasets except A0 and AL0. Another gene known for being a repressor of flowering locus C, AT3G18990 was also up regulated in P.
enysii in datasets PL0 and PL1. a total noob Mainly because of their larger altitude, P. enysii populations are covered with snow for the most part of the year leaving a relatively short timeframe to produce flowers and seeds. While plants for the two species had been collected concurrently of yr, and just before flowering, up regulation of genes that induce flowering in P. enysii may be indicative of an earlier flowering time for this species. Analysis of homeologous gene copies We investigated 379 homeologous gene pairs and 5 gene triplets consisting of two homeologues and 1 paralogue for copy unique expression. Of those 773 genes, 245 and 136 were up regulated in P. enysii and P. fastigiatum, respectively, whereas 392 copies were not differen tially expressed.
For fifty five and 19 pairs, both copies were up regulated in P. enysii and P. fastigiatum, respect ively. For 101 and 64 pairs, one particular copy was up regulated in P. enysii and P. fastigiatum, respectively, whereas the other copy was not differentially expressed. For 33 pairs 1 copy was up regulated in one species while another was natural compound library up regulated while in the other species, Myrosinase associated proteins which perform in glucosinolate metabolic process had a variable number of homeologous copies from the P. fastigiatum EST library. none for AT1G54000 and AT1G54010, one for AT1G54020, AT3G14220, and AT3G14225, and 3 for AT1G54030, Two with the MVP1 sequences are most comparable to a homologue on chromosome 1 in a. thali ana however the third MVP1 sequence is most comparable to a gene within the Arabidopsis lyrata genome that precedes the ESM1 gene on chromosome three. Differential expression for some of the MyAP copies was observed. AT1G54020 was up regulated in P. enysii whereas one of several homeologous MVP1 was up regulated in P. fastigiatum. The highest amount of tags mapped on the third MVP1 copy that was also up regulated in P. fastigiatum. Myrosinase TGG2, and that is regarded to interact with MVP1, was not present while in the P.