Eleven Seliciclib genes (Bcat2, Ddx54, Efnb2, Prickle2, Ptpro, Srms, Tcf4, Tnk2, Trio, Wscd1, and Zscan22), identified from unique RAMs, were observed in both groups of mice (Table 3). Five genes were identified from RAMs whose methylation statuses either clearly increase (Bcat2: M464-468 and Zscan22: H238�C239) or clearly decrease (Ddx54:B312-315, Ptpro: B341�C342 and Wscd1: M358�C359) in both the B6C3F1 and CAR WT mice. Three genes were identified from RAMs whose methylation statuses are clearly opposite (e.g., increases in one group and decreases in another) in at least 1 B6C3F1 group and one CAR WT group: Ddx54 (M315-317), Tcf4 (H200) and Tnk2 (M275�C276). Prickle2 (B310-312), and Tcf4 (B200) were identified from RAMs in the B6C3F1 whose methylation statuses increase at 2 weeks, decrease at 4 weeks, and either increase (Tcf4) or decrease (Prickle2) in the CAR tumor tissue.

Wscd1 (B357�C358) was identified from RAMs in the CAR precancerous and tumor tissue whose methylation statuses increase and decrease, respectively, and also increase in the B6C3F1 at 4 weeks. Finally, Efnb2 (M564�C566), Prickle2 (H310-312), Ptpro (H340�C343), Srms (M202�C206), and Trio (M564�C566) were identified from a RAM in at least one group whose methylation status is unclear after ��combining�� multiple RAMs (i.e., two RAMs formed in the same group and the methylation changes are opposite in direction), and thus, it is uncertain whether the altered methylation patterns
Rotavirus can lead to severe life-threatening gastroenteritis in infants and young children worldwide.

Unlike many other enteropathogens, rotavirus affects children in both developed and developing countries (1-2) and is not associated with socioeconomic factors. However, the risk of dying from severe rotavirus-associated infection is higher in lower-income countries due to various factors, including limited access to healthcare facilities, malnutrition, earlier onset of infection, and mixed infections involving rotavirus and other pathogens. Rotavirus infects most children by three years of age, and prevention of rotavirus spread is virtually impossible, even in environments with the highest hygiene measures Dacomitinib (2). Improvements in the availability of safe water and sanitation, access to oral rehydration therapy, and higher vaccine coverage have reduced the overall burden of gastroenteritis in children (3-4). From 1980 to 2002, Brazil showed a decrease by 91.5% in mortality due to gastroenteritis among children aged less than five years (3-4). During the same period, the proportion of infant deaths attributed to gastroenteritis fell from 41% to 4.7%, but with a range of 2.1% in the Southeast region to 7.8% in the Northeast (3-4). Infant mortality decreased from 85.2 per 1,000 children in 1980 to 25.