DnrO binds 18 bp upstream of the dnrN promoter to activate

it (Jiang & Hutchinson, 2006). DnrN in turn activates dnrI, a key regulator that activates all structural and resistance genes. Inactivation of any one of these genes results in complete blockade of DNR biosynthesis (Otten et al., 2000). Transcription of dnrO is driven by three promoters, Op1, Op2 and Op3, positioned, respectively, at 4, 315 and 365 bp ahead PD-0332991 concentration of the start codon. The DnrN promoter is positioned between Op1 and Op2 in the opposite strand. The binding region of DnrO (37 bp) overlaps the Op1 promoter and thereby autoregulates (represses) itself (Jiang & Hutchinson, 2006). Thus the binding of DnrO offers two functions: activation of dnrN and repression of its own transcription. DNA-binding drugs like DNR can interfere with the functions of vital enzymes such as DNA polymerase, RNA polymerase, topoisomerases and nucleases (Straney & Crothers, 1987; Woynarowski et al., 1989). These drugs can block the progress of DNA replication and transcription,

and inhibit the binding activity of DNA-binding proteins (Straney & Crothers, 1987). DNR has very high affinity for DNA (Cullinane et al., 1994) and is known to intercalate between GC-rich sequences. One important activity of DNR is the inhibition of mammalian topoisomerase II activity by inhibiting double-strand breakage and re-ligation (Drlica & Franco, 1988; Pommier et al., 1995). For all antibiotic-producing organisms, one or more self-resistance mechanisms confer protection to the producing organism from toxic effects (Cundliffe, 1989). These organisms escape cytotoxicity by different mechanisms such as drug inactivation,

target site modification, JQ1 drug efflux and drug sequestration (Cundliffe, 1989). Many secondary metabolites inhibit or repress their own biosynthesis (Martin & Demain, 1980). Feedback inhibition is one of the vital mechanisms Carnitine palmitoyltransferase II employed by antibiotic-producing organisms to maintain optimum intracellular drug levels. End product inhibition has been observed in several antibiotic-producing Streptomyces such as Streptomyces alboniger (Sankaran & Pogell, 1975), Streptomyces venezulae (Shaw & Leslie, 1991) and Streptomyces fradiae (Baltz & Seno, 1988), which produces puromycin, chloramphenicol and tylosin, respectively. The binding of the polyketide antibiotic jadomycin to activator protein JadR1 at the N-terminal domain restrains binding of JadR1 to biosynthetic gene promoters (Wang et al., 2009). Similarly, in Streptomyces coelicolor, undecylprodigiosin inhibits RedZ, a key transcriptional regulator involved in its own biosynthesis (Wang et al., 2009). Self-resistance to DNR is essential for S. peucetius due the toxic effects of this metabolite. Three genes, drrA, drrB and drrC, which are regulated by DnrI confer self-resistance to this organism. The accumulation of intracellular drug is curtailed by the efflux action of membrane-bound DrrA–DrrB heterodimer (Guilfoile & Hutchinson, 1991).