Dendritic cell (DC) as the most important
antigen presentation cell plays pivotal role in immune activation. Our previous study shows that intestinal lamina buy A-769662 propria DC (LPDCs) from PI-IBS mouse model induces CD4+T lymphocyte differentiated to Th17 and activates it. This study tested the hypothesis that LPDCs may contribute to intestinal mucosal immune activation and visceral hypersensitivity in the development of PI-IBS mouse model. Methods: Visceral hypersensitivity was induced by Trichinella spiralis infection in mice. LPDCs were isolated and purified by intestine digestion and magnetic label-based technique. Normal mice following adoptive transfer of 106 LPDCs from PI-IBS mouse by tail vein injection was sacrificed 120 hours later. HE staining of the small intestine was performed. Visceral sensitivity is assessed
by abdominal withdrawal reflex (AWR). Expression of INF-γ, IL-4 and IL-17 in ileum and proximal colon were determined by western blotting. Results: 1) Compared with control, there is neither significant hyperemia and edema nor lymphocytic infiltration in the small and large bowel after adoptive transfer of LPDCs; 2) At 40, 60 mmHg, the AWR scores of adoptive transfer group were higher than that Mitomycin C in vitro in the control group (p < 0.05); 3) In the ileum, IL-17 after adoptive transfer (1.32 ± 0.22) was higher than that of control (0.95 ± 0.35, p < 0.05) while IL-4 was lower. In the comparison of control group, there were obvious changes
in proximal colon. No difference was observed in INF-γ between control and adoptive transfer group. Conclusion: Adoptive transfer of LPDCs from PI-IBS mouse model result in visceral hypersensitivity and increase proinflammatory cytokines. LPDCs play an important role in intestinal mucosal immune activation and visceral hyper sensitivity. Key Word(s): 1. PI-IBS; 2. LPDCs; 3. Adoptive transfer; 4. Visceral sensitivity; Presenting Author: XUHONG YU Corresponding Author: XUHONG YU Affiliations: The First Affiliated Hospital of Harbin Medical University Objective: To evaluate the clinical and endoscopic MCE公司 characteristics of abdominal type allergic purpura in adult patients as the evidences in early diagnosis. Methods: The clinical and endoscopic characteristics of 20 patients with abdominal type allergic purpura were analyzed retrospectively. Results: There is 20% who have remote cause; All the patients complained of the leader symptoms were paroxysmal abdominal colic, the occult blood test was positive in all patients. Purpura was found in 2–10 days after the presentation of abdominal pain. Endoscopy found hyperaemia, edema, bleeding spots, erosion and ulcer in gastroenterologic mucosa. Duodenal, ileum and caecum had more severe mucous lesions. The clinic misdiagnosis rates were 90%.