Conclusions PtdGro biosynthesis is not coupled to its GSK872 order utilization leading to the accumulation of pathway intermediates. The synthesis of cardiolipin significantly increased revealing a stress response to liberate glycerol-PO4 for PtdGro synthesis. Acyl-ACP accumulation correlated with a decrease in fatty acid synthesis. However, the regulation of
fatty acid synthesis was not stringent enough to prevent the accumulation of intracellular fatty acids. Acknowledgement This work was supported by www.selleckchem.com/products/ly2874455.html National Institutes of Health Grant GM034496, Cancer Center Support Grant CA21765 and the American Lebanese Syrian Associated Charities. References 1. Zhang Y-M, Rock CO: Membrane lipid homeostasis in bacteria. Nat Rev Microbiol 2008, 6:222–233.PubMedCrossRef 2. Cronan JE Jr, Rock GDC941 CO: Chapter 3.6.4. Biosynthesis of membrane lipids. In Eco-Sal-Escherichia coli and Salmonella typhimurium: cellular and molecular biology. Edited by: Böck I, Curtis RIII, Kaper JB, Karp PD, Neidhardt
FC, Nyström T, Slauch JM, Squires CL, Ussery D. Washington, DC: ASM Press; 2008. [Online] http://www.ecosal.org 3. Yao J, Rock CO: Phosphatidic acid synthesis in bacteria. Biochim Biophys Acta 1831, 2013:495–502. 4. Parsons JB, Rock CO: Bacterial lipids: Metabolism and membrane homeostasis. Prog Lipid Res 2013, 52:249–276.PubMedCrossRef 5. Heath RJ, Jackowski S, Rock CO: Guanosine tetraphosphate inhibition of fatty acid and phospholipid synthesis in Escherichia coli is relieved by overexpression of glycerol-3-phosphate acyltransferase ( plsB ). J Biol Chem 1994, 269:26584–26590.PubMed 6. Magnusson LU, Farewell A, Nystrom T: ppGpp: a global regulator in Escherichia coli . TIM 2005, 13:236–242. 7. Voelker TA, Davies HM: Alteration of the specificity and Inositol oxygenase regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J Bacteriol 1994, 176:7320–7327.PubMed 8. Jiang P, Cronan JE Jr: Inhibition of fatty acid synthesis
in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J Bacteriol 1994, 176:2814–2821.PubMed 9. Cho H, Cronan JE Jr: Defective export of a periplasmic enzyme disrupts regulation of bacterial fatty acid synthesis. J Biol Chem 1995, 270:4216–4219.PubMedCrossRef 10. Heath RJ, Rock CO: Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli . J Biol Chem 1996, 271:1833–1836.PubMedCrossRef 11. Heath RJ, Rock CO: Inhibition of b-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli . J Biol Chem 1996, 271:10996–11000.PubMedCrossRef 12. Davis MS, Cronan JE Jr: Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein. J Bacteriol 2001, 183:1499–1503.PubMedCrossRef 13. Lu Y-J, Zhang Y-M, Grimes KD, Qi J, Lee RE, Rock CO: Acyl-phosphates initiate membrane phospholipid synthesis in gram-positive pathogens.