At a concentration of 108  M. anisopliae spores/g, Trichostatin A an average of 12.3 ± 2.0 termites remained in the treated sand tubes while 23.0 ± 5.9 remained in the controls, but the difference was not significant. With some treatments, ex. I. fumosorosea and M. anisopliae in soil and sawdust, more termites remained in treated tubes after 24 h exposure than in control tubes, but none of the treatments

was significantly different from its respective control. Based on these data the fungi I. fumosorosea and M. anisopliae were shown to not be repellent to FST in sand, soil or sawdust. Table 1 Mean (±SEM) number of C. formosanus in a MEK162 in vitro paired choice test where tubes were filled with substrate treated with fungal spores at the indicated concentrations, after 24 h exposure   Number of termite in tubes Treatment Treated Control I. fumosorosea 10 6 spores/g Sand 36.3 ± 13.5a* 60.2 ± 17.3a Soil 96.1 ± 11.1a 77.4 ± 10.6a Sawdust 92.5 ± 9.6a 72.8 ± 10.2a I. fumosorosea 10 8 spores/g Sand 46.0 ± 6.5a 50.8 ± 4.5a Soil 71.3 ± 16.0a 82.7 ± 17.1a Sawdust 49.3 ± 9.8a 56.1 ± 9.7a M. anisopliae 10 6 spores/g Sand 23.9 ± 5.5a 45.0 ± 13.0a Soil 82.3 ± 7.4a 76.0 ± 7.0a Sawdust 93.4 ± 9.2a 62.7 ± 9.3a M. anisopliae 10 8 spores/g Sand 12.3 ± 2.0a 23.0 ± 5.9a Soil 78.3 ± 12.6a 77.6 ± 12.8a Sawdust 31.0 ± 3.9a Selleck PS341 36.5 ± 4.5a * Values with the same letter

are not significantly different, P ≤ 0.05. When termites were exposed to B. thuringiensis strain 33679 the effect of both cells and spores was determined. All treatments were applied at a concentration of 109 propagules/g. With cells in sand or soil, the treated tube values were not significantly different from the controls (Table 2). With cells in sawdust, the difference was highly significant with only 29.3 ± 6.6 termites remaining

in the treated tubes compared with 130.8 ± 9.6 in the control tubes (Paired choice t-test). These values indicated that the B. thuringiensis cells were strongly repellent to FST in sawdust. FST were also exposed to a B. thuringiensis culture in which the cells had formed spores due to nutrient deprivation. Neither the soil nor sawdust Montelukast Sodium treatments were significantly different from the respective controls, indicating that B. thuringiensis in these treatments was not repellent to FST. B. thuringiensis was also tested for its effect on FST as a mixture of cells and spores. The culture was incubated in media with a diluted nutrient source and the formation of spores was observed microscopically over time. The termites were exposed when the culture was as close as possible to 50% vegetative cells and 50% spores. In sand, the cell/spore treatment resulted in significantly more termites remaining in the control tubes compared with the treated tubes. Neither the soil or sawdust treatments were significantly different from the controls. Table 2 Mean (±SEM) number of C.