70a and b). Peridium 40–55 μm wide at the sides, up to 70 μm thick at the apex, thinner at the base, comprising two cell types, outer layer composed of small heavily pigmented thick-walled cells of textura angularis, cells 2–5 μm diam., cell wall 2–3 μm thick, apex cells smaller and walls thicker, inner layer composed of lightly pigmented or hyaline thin-walled cells of textura angularis, 5–7 μm diam., wall 1.5–2 μm thick, merging with pseudoparaphyses (Fig. 70c). Hamathecium of long cellular pseudoparaphyses, 2–3 μm broad, septate, anastomosing or branching not observed (Fig. 70e). Asci 150–195 × 8–12.5 μm (\( \barx = 169.5 \times 10.7\mu m \), n = 10), 8-spored, bitunicate, fissitunicate dehiscence
not observed, cylindrical but CB-5083 research buy narrowing towards the base, with a short, furcate pedicel which is 10–25 μm long, ocular chamber not observed (Fig. 70d and e). Ascospores 110–160 × 2.5–4 μm (\(
check details \barx = 135.3 \times 3\mu m \), n = 10), filamentous, narrower toward the lower end, pale brown, 22–30-septate, PF-02341066 in vitro separating into two partspores from the middle septum, from the breaking point the second cell of each partspore enlarged. Anamorph: none reported. Material examined: GERMANY, near Kassel, on dead stem of Cirsium arvense (L.) Scop., Spring 1853 (BPI-629021, type). Notes Morphology Ophiobolus was established by Reiss (1854) as a monotypic genus represented by O. disseminans based on its “Perithecia discreta, ostiolis prominentibus: sporae ascis inclusae, binatae, filliformes, multiseptatae”. A broad generic concept was adopted for the genus by Holm (1948) and Müller (1952). Shoemaker (1976) surveyed Canadian species of Ophiobolus using the broad concept of Holm (1948) and Müller (1952). A narrower generic concept was used by Holm (1957), which only included Olopatadine species with ascospores separating into two halves. Holm (1957) assigned species with enlarged ascospore
cells to Nodulosphaeria, and those with long spirally coiled ascospores to Leptospora (Shoemaker 1976). This left only three species accepted under Ophiobolus (Holm 1957), although this concept has rarely been followed with new species recently being described (Raja and Shearer 2008). Walker (1980) provided a detailed description from the type material and dealt with many species of scolecospored fungi that had been placed in Ophiobolus by Saccardo (1883). Thus, currently several Ophiobolus sensu lato species are separated into Acanthophiobolus, Entodesmium, Leptosphaeria and Leptospora. Ophiobolus sensu lato contains about 300 species names (Sivanesan 1984; http://www.mycobank.org/, 04/02/2009). Phylogenetic study Ophiobolus fulgidus (Cooke & Peck) Sacc. (as Leptosphaeria fulgida (Cooke & Peck) M. E. Barr in Dong et al. 1998) lacks support in the clade of Leptosphaeriaceae (Dong et al. 1998). We expect it may closely related to Phaeosphaeriaceae.