66 In contrast,

soluble PD-1 binding to B7-H1 and/or B7-DC on DCs inhibited their maturation and induced IL-10 production, thus promoting a more suppressive phenotype.67 Together, these results demonstrate Metformin that signals delivered through B7-H1/-DC affect APCs; however, the downstream effect of these signals vary, and it is unclear what dictates the outcome of reverse signals because these effects have been studied with isolated cell populations in vitro. Decidual stromal cells express class I MHC and can express class II MHC in vitro after treatment with proinflammatory cytokines, therefore raising the possibility that they can present antigen. These cells lack the costimulatory molecules B7-1 and B7-2 but express B7-H1 and B7-DC.25,68In vitro, it was found that decidual stromal cells could stimulate an allogeneic reaction from unrelated CD4+ T cells, a response that was kept in check by B7-H1 and B7-DC.68 The potential role of these cells in controlling an immune response during pregnancy poses an interesting question that warrants further investigation. For example, they might play a role in local control of T-cell responses to infection by presenting foreign antigen, with the inhibitory B7s tailoring cytokine production to an appropriate balance for the maternal–fetal environment.

Whether or not these cells could present fetal antigen and play BMN 673 research buy a role in tolerance to the fetus has not yet been investigated. In the human placenta, B7-H1 is expressed

by all trophoblast populations.25,69 Its expression is increased during placental development, possibly attributed to the increases in oxygen levels from the first to the second trimester concurrent with the influx of maternal blood to the placenta.70 In addition, syncytiotrophoblast expresses more B7-H1 than does cytotrophoblast, its immediate precursor. Treatment with epidermal growth factor in vitro, which promotes syncytialization, recapitulates this effect by the post-transcriptional mechanism of shifting mRNA to the polysomes.44 This mechanism of regulation is in line with learn more B7-H1 expression being regulated post-transcriptionally, which is likely, as mentioned previously, based on the broad distribution of its mRNA compared with the more restricted expression of B7-H1 protein. Although B7-H1 is occasionally expressed by placental macrophages (M. Petroff, unpublished observations), the syncytiotrophoblast and extravillous trophoblast cells are the major sources of the protein at the human maternal–fetal interface. Our laboratory has identified PD-1 expression on CD4+ and CD8+ T cells, as well as CD4+ CD25+ FoxP3+ TRegs isolated from the decidua. Using a human choriocarcinoma cell line transfected with B7-H1, we showed that B7-H1 promotes Th2 but suppresses Th1 cytokine production by decidual lymphocytes.71 These results highlight an interesting conundrum regarding B7-H1 function in trophoblast cells.