5% of the contigs (Table 2). These data generated from the mantle of P. maximus form a valuable addition to those generated from hemocytes of the same species ( Pauletto et al., 2014) and, in a
more general context, to the transcriptomes generated for other molluscs including the Yesso scallop Patinopecten yessoensis ( Hou et al., 2011), Mytilus galloprovincialis ( Craft et al., 2010), Laternula elliptica ( Clark et al., 2010), Meretrix selleck kinase inhibitor meretrix ( Huan et al., 2012), Ruditapes philippinarum ( Milan et al., 2011), Haliotis midae ( Franchini et al., 2011), several pearl oysters ( Huang et al., 2013) and the oyster genome data ( Zhang et al., 2012), thus increasing the sequence resource available for commercially important shellfish species and for researchers investigating shell deposition processes in molluscs. The sequence data for this transcriptome has been deposited in the GenBank SRA, accession number: SRP040427. The contigs, and the annotation for those contigs with a match of 1e − 10 and lower, are available from http://ramadda.nerc-bas.ac.uk/repository/entry/show/Polar+Data+Centre/NERC-BAS+Datasets/Genomics/. This study was funded by grants from the Région Bretagne, i.e. the
Pemadapt project (ref. 6368) and a doctoral fellowship to S.A. (Protmar project, ref. 6197). This study was also supported by a Natural Environment Research Council (NERC) grant to Lloyd Peck (NE/G018) and the British Antarctic Survey Polar Sciences for Planet Earth programme, which is also funded by the Natural Environment Research Council. Two French National Research Agency Selleck PF-01367338 (ANR) programmes also supported our research: COMANCHE (ANR-2010-STRA-010) and LabexMER (ANR-10-LABX-19-01). “
“Three congeners of the salmonid fish family with high commercial value were under study Sitaxentan to identify species-specific markers for a validated species determination: Oncorhynchus mykiss, Salmo salar and Salmo trutta. The latter ones are common in Polish marine waters, whereas O. mykiss is only represented in this region in hatcheries. Difficulties in species identification may result in non-sustainable
salmon fisheries leading to imbalances in the ecosystem. The development of easy tests by means of molecular markers will therefore help in monitoring fishery activities as well as natural stock development. Single nucleotide polymorphisms (SNPs) have been used for ecological and conservational studies and have proven very useful for differentiating individuals, populations and species. A species-specific SNP-microarray initially comprised of 15163 loci was constructed and then optimized to 7000 markers for functional genes of S. salar in CIGENE, Norway. It has been used in studies of differences between farmed and wild Atlantic salmon ( Karlsson et al., 2011), genetic architecture of North Atlantic populations ( Bourret et al., 2013), and characterization of SNPs in S. trutta populations from the Southern Baltic Sea ( Drywa et al., 2013).