2 Pharmacokinetics Plasma concentration–time

curves of TRA, bendamustine, M3, M4, and HP2 during 24 hours after the start of the 14C-bendamustine infusion Etomoxir purchase are presented in Fig. 2. HP2 dihydroxy bendamustine, M3 γ-hydroxy-bendamustine, M4 N-desmethyl-bendamustine, TRA total radioactivity Table 2 Plasma pharmacokinetic Batimastat solubility dmso parameters for total radioactivity, bendamustine, and the metabolites γ-hydroxy-bendamustine, N-desmethyl-bendamustine, and dihydroxy bendamustine following an intravenous 60-minute infusion of 120 mg/m2 of 14C-bendamustine hydrochloride Parameter Patient Mean [SD] 1 2 3 4 5 6 BSA (m2)   EPZ015666 manufacturer 2.17 1.84 1.85 1.6 2.05 1.7 NC Dose (mg)a   233 198 197 172 215 182 NC TRA (bendamustine equivalents) Cmax (μg/mL) 6.88 12.4 9.31 12.1 8.54 12 10.2 [2.29] AUC∞ (μg·h/mL) 904 1,147 1,504 695 1,403 1,571 1,204 [351] t½ (h) 225 110 261 171 222 193 197 [52.5] Vss (L) 81.2 27.4 48.3 59.2 49.6 31.3 49.5 [19.6] CL (mL/min) 4.27 2.89 2.16 4.13 2.56 1.92

2.99 [1] Bendamustine Cmax (μg/mL) 3.25 7.48 4.2 8.19 3.6 5.2 5.32

[2.07] AUC∞ (ng·h/mL) 3,963 Carnitine palmitoyltransferase II 10,619 4,906 8,041 4,487 6,371 6,398 [2,543] t½ (h) 0.57 0.96 0.58 0.86 0.45 0.46 0.65 [0.21] Vss (L) 27.1 15.3 24.4 10.7 27.5 15.5 20.1 [7.1] CL (mL/min) 977 313 666 358 800 476 598 [262] CLR (mL/min) 14.3 16.1 11 6.6 29.9 28.5 17.7 [9.5] M3 Cmax (ng/mL) 644 264 714 1,125 550 816 685 [286] AUC∞ (ng·h/mL) 829 389 975 1,428 792 1,137 925 [351] t½ (h) 3.58 0.82 1.41 2.14 1.09 1.12 1.69 [1.03] M4 Cmax (ng/mL) 38.7 29.8 50.1 87.9 28.5 117 58.7 [36.1] AUC∞ (ng·h/mL) 59 61 81 119 43 135 83 [37] t½ (h) 0.48 0.8 0.48 0.44 0.45 0.45 0.52 [0.14] HP2 Cmax (ng/mL) 35 73.3 43.2 53.1 40.8 81.4 54.5 [18.8] AUC∞ (ng·h/mL) NC NC 188 153 215 NC 185 [31] t½ (h) NC NC 15.4 14.1 23.8 NC 17.8 [5.3] AUC ∞ area under the plasma concentration–time curve from time zero to infinity, BSA body surface area, C max maximum observed plasma concentration, CL clearance, CL R renal clearance, HP2 dihydroxy bendamustine, M3 γ-hydroxy-bendamustine, M4 N-desmethyl-bendamustine, NC not calculable, SD standard deviation, TRA total radioactivity, t ½ elimination half-life, V ss apparent volume of distribution at steady state aBendamustine free base (mg) The Cmax values of TRA, bendamustine, and HP2 were typically observed in the first sample after completion of the infusion (median time to reach Cmax [tmax] 1.10 hours), and the median tmax durations of M3 (1.26 hours) and M4 (1.28 hours) were slightly longer.