This should improve its effectiveness both as a probiotic and as a treatment for diarrhea. Acknowledgments Our laboratory is supported by the following grants awarded to N. Austriaco: NIGMS R15 GM094712, NSF MRI-R2 0959354, NIH Grant 8 P20 GM103430-12 to the Rhode Island INBRE Program for student training, and a CAFR faculty research grant from Providence College. The funders had no role in study design, data collection see more and analysis, decision to publish, or preparation of the manuscript. Non nisi te, Domine. Electronic supplementary material Additional file 1: Differentially Regulated Genes

in S. boulardii Cells Cultured in an Acidic Environment. S. boulardii genes showing 4-fold or greater increase (up-regulated) or decrease (down-regulated) expression in response to an acidic environment. This data has been submitted to the Gene Expression Omnibus (GEO) at the NCBI with accession number, GSE43271. (XLS 286 kb) (XLS 286 KB) References 1. FAO/WHO: Guidelines for the Evaluation of Probitics in Food. Food and Agriculture Organization

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