One unit was defined as the
amount of enzyme that releases a sufficient amount of azo dye from azocoll substrate to produce an increase in A 520 of 0.001 per min at 37°C, pH 7.5. Murine model of thermal injury The experiments were conducted as previously described [61]. Animals were treated in accordance with Protocol 96020 approved by the Institutional Animal Care and Use Committee at Texas Tech University Health Sciences Center in Lubbock, TX. Statistical analyses Statistical analyses were done using GraphPad InStat 3.06 (GraphPad Software, San Diego, CA). One-way ANOVA with the Tukey-Kramer multiple Avapritinib ic50 comparisons post-test was used to determine significant differences across time. The two-tailed t-test was used to compare pairs of strains containing different plasmids. Acknowledgements We thank Susan West (PAOΔvfr, pKF917, and pUCP19) and Barbara H. Iglewski (PAO-R1) for their kind provision of strains or plasmids. We also thank Uzma Qaisar for assistance with the qRT-PCR and Joanna E. Swickard for critical reading of the manuscript. Electronic supplementary material Additional file 1: Oligonucleotides used in this study. (PDF 89 KB) Additional file 2: Amino acid homology of the predicted
PA2783 protein endopeptidase domain with other bacterial proteins. (PDF 18 KB) Additional file 3: The predicted AZD5582 datasheet PA2783 protein carries two carbohydrate-binding modules. Interrogation of the non-redundant databases at NCBI
(http://www.ncbi.nlm.nih.gov/; accessed 06/19/2013) using BLASTP revealed homology with the CBM_4_9 family (Cdd:pfam02018) of diverse CHO-binding proteins. CHO-binding domain I (A) and domain II (B), have different aa sequences but both were strongly homologous to the two CHO-binding modules of the Pseudomonas mendocina (Pmendo) carbohydrate-binding CenC domain-containing protein and the Ni,Fe-hydrogenase I small subunit of Hahella Glycogen branching enzyme chejuensis (Hcheju). For the pfam, identical aa are indicated by * and similar aa by ^; for bacterial proteins, identical aa are shown in red, similar aa in blue, and non-similar aa in black; Pmucil, Paenibacillus mucilaginosus; Clen-1 and Clen-2, Cellulosilyticum lentocellum CHO-binding selleck chemicals llc domains I and II. Percentages of aa identity and similarity are shown in Additional file 4. (PDF 392 KB) Additional file 4: Amino acid homology of the predicted PA2783 protein carbohydrate-binding domains I and II with other bacterial proteins. (PDF 16 KB) References 1. Branski LK, Al-Mousawi A, Rivero H, Jeschke MG, Sanford AP, Herndon DN: Emerging infections in burns. Surg Infect (Larchmt) 2009,10(5):389–397.CrossRef 2. Fishman JA: Infections in immunocompromised hosts and organ transplant recipients: essentials. Liver Transpl 2011,17(Suppl 3):S34-S37.PubMedCrossRef 3. Lyczak JB, Cannon CL, Pier GB: Lung infections associated with cystic fibrosis. Clin Microbiol Rev 2002,15(2):194–222.PubMedCrossRef 4.