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group vs. Ad5 group). (C) In the Ad5-HIF-1alpha group, the growth of cells was promoted after blockade of SOCS1 by Ad5-siSOCS1 (* p < 0.01 Ad5- HIF-1alpha/siSOCS1 group vs. Ad5-HIF-1alpha NVP-BSK805 solubility dmso group). (D) In the Ad5-si HIF-1alpha group, the growth of cells was slowed after co-transfection with SOCS1 (* p < 0.05 Ad5-siHIF-1alpha/SOCS1 group vs. Ad5-siHIF-1alpha group). (E) In the Ad5-HIF-1alpha group, the growth of cells was slowed from day 5 to day 8 as the growth curve moved right after co-transfection with SOCS1 (* p < 0.05 Ad5-HIF-1alpha group vs. click here Ad5-HIF-1alpha/SOCS1

group). Figure 5 We used the tunel stain to investigate the effect of HIF-1alpha and SOCS1 on cell apoptosis and the apoptosis rate was calculated in all the experimental groups. (A) The background was clear and the apoptotic NCI-H446 nucleuses were stained yellow and normal nucleuses were stain blue(tunel stain × 400) (B) The effect of HIF-1alpha

and SOCS1 on apoptosis of SCLC cells after transfection for 8 d (*p < 0.05 Ad5-HIF-1alpha/siSOCS1 group vs. Ad5-HIF-1alpha group; **p < 0.05 Ad5-HIF-1alpha/siSOCS1 group vs. Ad5-siSOCS1 group; ***p < 0.01 Ad5-si HIF-1alpha/SOCS1 group vs. Ad5-siHIF-1alpha group; ****p < 0.05 Ad5-si HIF-1alpha/SOCS1 group vs. Ad5-SOCS1 group). Discussion Tissue hypoxia is critical in the process of tumor formation. Activation of HIF-1 alpha, an important transcription factor that is expressed in response to hypoxia, selleckchem is a common feature of tumors and is generally more pronounced in aggressive solid tumors such as SCLC and can even be an independent predictor of prognosis in certain types of cancer [9, 10]. To characterize the molecular mechanisms involved in the carcinogenesis, progression and prognosis of SCLC which are regulated by HIF-1 alpha and identify genes to be applied as novel diagnostic markers or for development of gene targeted therapy, we applied cDNA microarray profile analysis and integrated the results of gene expression profiles of the hypoxia, HIF-1 alpha and siHIF-1 alpha groups. In this way, we could eliminate the effects on gene expression by others factors involving the hypoxic microenvironment and stringently screened out the genes regulated by HIF-1alpha.