Together, these results suggest that there may be a complex relationship between the homeostatic and inflammation-associated production of LPC by APCs and the resulting activation

of iNKT cells and other CD1d-restricted subsets. Another mechanism by which iNKT cell responses may be physiologically modulated is via the regulation of CD1d cell surface expression levels. It has been shown that CD1d is up-regulated on murine macrophages following exposure to IFN-γ and one other signal, which can come from inflammation-associated cytokines Sirolimus cost such as tumour necrosis factor (TNF)-α, or from microbial infection of the macrophage, or simply from exposure to microbial products.131 As the up-regulated CD1d expression was associated with enhanced iNKT cell activation, this observation suggests that infected and non-infected bystander macrophages might similarly stimulate increased iNKT cell responses. Expression levels of CD1d on human myeloid DCs have been found to be regulated by a type of nuclear hormone receptor called peroxisome proliferator-activated receptor γ (PPAR-γ). Receptors of this family are known to regulate C59 wnt clinical trial the expression of genes involved in energy management (e.g. genes relating to lipid storage, metabolism and transport), as well as genes involved

in inflammatory processes and wound healing.132 Like other receptors of this type, PPAR-γ resides in the cytoplasm in an inactivated state until it binds a specific ligand, generally a hydrophobic or lipidic molecule, whereupon it translocates to the nucleus and acts as a transcription factor for genes that include the appropriate response element sequences.132 Szatmari et al.133 have shown that exposure of DCs to oxidized low-density lipoprotein (LDL) results in the activation of PPAR-γ and transactivation of genes that turn on the retinoic acid synthesis pathway. The resulting production of all-trans retinoic acid eventually leads to activation of retinoic acid receptor-α

(RAR-α), which in turn transactivates CD1d mRNA synthesis.133 Thus, CD1d expression levels are directly modulated by RAR-α, but this pathway can be indirectly activated by exposure to PPAR-γ ligands, including lipids associated with oxidized LDL. As oxidized LDL is an inflammation-associated danger signal Interleukin-2 receptor that may be generated even in the absence of a pathogenic microbial challenge, these results suggest that CD1d expression by myeloid APCs, and consequently NKT cell activation, may be linked to broad pathways of endogenous inflammatory activation. Investigations over the last 15 years have revealed a surprising complexity and variety to the range of interactions between iNKT cells and myeloid APCs. It seems that iNKT cells can induce DCs to become highly stimulatory, but they can also cause them to gain a more tolerizing phenotype.