There is some, perhaps rather controversial, evidence that CD8+ T cells, when first activated to proliferate, require an asymmetric cell division to provide one daughter that will generate MK-8669 manufacturer the effector cell lineage while the other daughter gives rise to memory cells.[71] If that is true, it is tempting to speculate that TORC2, which seems to have an evolutionary conserved function

in controlling cell shape and polarity,[16, 72] may regulate asymmetric cell divisions and the subsequent lineage decisions of both CD4+ and CD8+ T cells in ways we do not yet understand. The mTOR pathway can therefore be thought of as the fulcrum that balances the different requirements of T cells in tolerance compared with inflammation (Fig. 4). During inflammation, effector T-cell differentiation dominates, which is associated with extracellular ATP and a ready availability of amino acids that, in turn, drive mTOR activation, cell proliferation and glucose metabolism. In contrast, tolerance is maintained by an excess of regulatory T cells, associated with a TGF-β-induced expression of CD39 and CD73, and conversion of extracellular ATP to adenosine. Tolerance within tissues is also associated

with the up-regulation of many different enzymes that consume many, if not all, of the essential amino acids. Under these conditions, mTOR is inhibited, FOXP3 induction is promoted in naive T cells (i.e. infectious AZD9291 concentration tolerance), and

both iTreg and nTreg cells may have a competitive advantage to accumulate relative to effector GNA12 T cells. However, under conditions of mTOR inhibition, Treg cells may not be optimally functional, and it may only be in response to inflammation and mTOR activating conditions that the Treg cells acquire the full suppressive potential. The author has no conflict of interests. “
“Chronic periodontitis is the most common chronic inflammatory disease and has been associated with an increased risk for serious medical conditions including cardiovascular disease (CVD). Endotoxin (lipopolysaccharide), derived from periodontopathogens, can induce the local accumulation of mononuclear cells in the inflammatory lesion, increasing proinflammatory cytokines and matrix metalloproteinases (MMPs), resulting in the destruction of periodontal connective tissues including bone. In this study, we show that doxycycline, originally developed as a broad-spectrum tetracycline antibiotic (and, more recently, as a nonantimicrobial therapy for chronic inflammatory periodontal and skin diseases), can inhibit extracellular matrix degradation in cell culture mediated by human peripheral blood-derived monocytes/macrophages. The mechanisms include downregulation of cytokines and MMP-9 protein levels and the inhibition of the activities of both collagenase and MMP-9.