The extents of fresh water plumes or upwelling extents were determined by the positions of thermal fronts. These fronts were mapped by spatial domain filtration (3 × 3 window size) and calculating the gradient towards the local maximum of SST change, after previous median filtering to eliminate noise (Cayula and Cornillon, 1992 and Belkin and O’Reilly, 2009). The frontal zone was assumed to be an elongated, at least 5 km long, group of pixels with Fluorouracil in vivo gradients over 0.2 °C km− 1. The project

commenced in 2008 and preliminary samples were collected from July to October. During this initial stage of the Ferry Box measurements a number of technical problems were encountered, one of the most annoying being severe fouling of the sensors by young forms of Mytilus trossulus and by Balanus spp.; malfunctioning of the WaterSam autosampler was also a common occurrence. The automatic measurements of temperature, salinity and chlorophyll

a showed a variability of environmental factors over the period from 11 July to 9 October 2008 ( Figure 2). Dissolved inorganic phosphate (DIP), oxygenated inorganic nitrogen (TO × N = NO3 + NO2), silicate, total phosphorus (TP) and total nitrogen (TN) were analysed in discrete seawater samples (TP and TN are not discussed here). Ammonia was not determined because the samples could not be treated with reagents EPZ-6438 in vitro immediately after sampling. Nutrient concentrations determined in discrete seawater samples depended on the station location and sampling date. Results from the off-shore station (GK4) are shown in Figure 3 to illustrate the observed fine changes in nutrient levels. From 7 July to 10 October 2008, chlorophyll a was measured in samples from discrete sampling stations on 11 occasions. The results showed considerable variability in chlorophyll

a concentrations, depending on the location of the sampling station and sampling date ( Figure 4). Phytoplankton species structure, abundance and biomass were determined in discrete samples on 3 occasions, between 7 and 28 July 2008 (Figure 5). The species structure showed considerable diversity (Figure 5). Flagellates were dominant in terms of both biomass and abundance, although there was also a marked presence of Dinophyceae in the biomass. The contributions of Cyanophyceae HSP90 and Bacillariophyceae to the biomass were considerable in the off-shore part of the ferry route. In fact, the biomass of the latter class consisted of a single diatom species – Actinocyclus octonarius. As for blue-green algae, the potentially toxic species Nodularia spumigena, accompanied by Aphanizomenon flosaquae, were dominant among the Cyanophyceae in general ( Figure 6), and Aphanothece paralleliformis was found to be dominant at a single station. The presence of nodularin was detected in discrete samples collected between 7 July and 13 August.