2 Iyengaria stellata (Børgesen) is classified as a brown algae or seaweed belongs to the family Scytosiphonaceae and class Phaeophyceae. 3 According to Silva, Basson & Moe, 1996 the type locality of

Iyengaria stellata is Dawarka, Gujarat, India. 4 Furthermore they found that the seaweed is geographically distributed in India, 5 Singapore. 6 Kuwait, Iran, 7 Papua New Guinea, 8 Pakistan, 9 Oman, 10 Saudi Arabia and South Africa. 11 Collection of seaweed can also be done from Karachi sea port (Manora, Paradise Point, Buleji, Hawkes Bay, and Cape Monze) and Baluchistan sea shores (Sur Bunder, Sonmiani, Gadani, Gawader and Jiwani). Spring and summer seasons are favorable for the growth of this seaweed at Karachi coast. Various studies on the composition of Iyengaria stellata have been conducted by different researchers ZD1839 Alectinib manufacturer and revealed the presence of notable constituents. Khan in 2000 carried out phytochemical

study on Iyengaria stellata and isolated saringosterol, loliolide, propyl-4-hydroxy benzoate and methyl-4-hydroxy benzoate. 12 Earlier researches on this alga have indicated the presence of amino acids, carbohydrates and vitamins. 13 and 14 Other research scholars have documented the occurrence of polysaccharides, 15 proteins, amino acids, lipids and mannitol. 16 Usmanghani, et al, analyzed Iyengaria stellata for its fatty acid constitution resulted in the presence of methyl-n-pentadecanoate, Mannose-binding protein-associated serine protease methyl hexadecanoate, methyl-n-heptadecanoate, methyl octadecanoate, methyl 9, hexadecenoate and methyl 9, octadecenoate. 17 According to another investigation cholesterol with another new metabolite stellatol was detected from the extract of Iyengaria stellata. 18 Elemental composition includes Ca, Cd, Cr, Cu, Fe, K, Mg, Na, Pb, and Zn. 19 Iyengaria stellata showed hypolipidemic activity, 20 ChE activity 21 haemagglutinic

activity, 22 antibacterial activity, antifungal activity, phytotoxic, insecticidal and nematicidal activity. 23 LC 50 of Iyengaria stellata was found to be 186 mcg. 24 Not enough scientific work has been done to determine the effect of Iyengaria stellata on hematological parameters. For the first time current research has been conducted to establish hematopoietic effect of Iyengaria stellata in an attempt to seek treatment against anemia. Prior to the initiation of the experimental work, collection of algae was done which was then identified by department of Botany, University of Karachi. Later drying followed by extraction was conducted to obtain the extract.18 Healthy albino rabbits of either sex weighing from 1500 to 2000 g were selected. Rabbits were selected as experimental animals because of several reasons like biochemical and histopathological changes produced in rabbits are comparatively similar as observed in humans.