There was no significant weight alteration in the mice before or after the experiments. However, an increase in weight was observed in group C when compared with group B after 28 d of experimentation . Histological examination At the end of the experiment, there was no significant difference in the macro appearance of the livers from mice in the three groups. The livers were pink, soft and their borders were even. Morphologically, the liver structure was intact, with few necrotic hepatocytes, limited inflammatory cell infiltration and fibrous tissue formation . HBV DNA content in serum was reduced by lamivudine, emodin and APS The use of real time PCR showed that lamivudine significantly decreased serum HBV DNA content after one week of administration, and this inhibitory effect lasted up to 21 d. HBV DNA content increased to the original level when lamivudine administration was stopped for one week. However, emodin and APS did not decrease serum HBV DNA content after 7 or 14 d of administration , but reduced HBV DNA content after 21 d of administration, and this inhibitory effect lasted up to day 28, one week after administration ceased . There was no alteration in serum HBV DNA content in mice from the control group.
Lamivudine and emodin APS reduced HBsAg and HBeAg levels in serum HBsAg and HBeAg levels in serum were determined using ELISA at day 28 of the experiment and showed that lamivudine and emodin APS significantly decreased HBsAg and Nilotinib selleckchem HBeAg levels in serum in the treated groups, compared with the control group . However, there was no significant difference in HBsAg and HBeAg levels between the lamivudine group and the emodin APS group . Lamivudine and emodin APS inhibited HBsAg and HBcAg expression in hepatocytes HBsAg and HBcAg expression in mouse liver tissue was also investigated using immunohistochemistry. HBsAg and HBcAg positive staining was brown or dark brown, and mainly localised within the cytoplasm. Both HBsAg and HBcAg were distributed throughout the liver tissue, especially in the portal area and around the central vein area . The positive ratios of HBsAg staining in hepatocytes were 80 , 75 and 80 , respectively, and the positive ratios of HBcAg staining were 55 , 45 and 50 , respectively.
buy Rucaparib There was no significant difference in the positive ratios of HBsAg and HBcAg staining in hepatocytes between these three groups . However, lamivudine and emodin APS decreased the positive staining of HBsAg and HBcAg in hepatocytes after analysis according to the IRS . DISCUSSION The transgenic mouse model used in this study was established by integration of HBV genome into mouse genome using a microinjection method, and it has been confirmed that HBV genes can be stably expressed, replicated and packaged in the mouse . The present study showed that administration of lamivudine for 3 wk significantly reduced serum HBV DNA content.